PRPF40B Stable Expression Cell Line (Hela)

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PRPF40B Stable Expression Cell Line (Hela)

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PRPF40B Stable Expression Cell Line (Hela)

PRPF40B Stable Expression Cell Line (Hela) Copy Product Info

Catalog#： YOE-L072

Size： 1*10^6

Culture method： 90%DMEM+10% FBS

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STR Report

Datasheet (Use Instruction)

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PRPF40B Stable Expression Cell Line (Hela)

PRPF40B Stable Expression Cell Line (Hela) Copy Product Info

Catalog#： YOE-L072

Size： 1*10^6

Culture method： 90%DMEM+10% FBS

Order

STR Report

Datasheet (Use Instruction)

Detailed Product Information

Cell Info

Catalog	YOE-L072	Transcript	NM_001002026.3
Cell name	PRPF40B Stable Expression Cell Line (Hela)	Species	Human
Gene Name	PRPF40B	Passage ratio	1：2-1：4
Cell Morphology	Epithelial-like, adherent	Antibiotic Concentration	Puro=1μg/ml
Culture method	90%DMEM+10% FBS	Mycoplasma test	negative

Catalog

YOE-L072

Cell name

PRPF40B Stable Expression Cell Line (Hela)

Gene Name

PRPF40B

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：2-1：4

Transcript

NM_001002026.3

Species

Human

Antibiotic Concentration

Puro=1μg/ml

Mycoplasma test

negative

Culture method

90%DMEM+10% FBS

Product Description

This gene encodes a WW-domain containing protein similar to yeast splicing factor PRP40. This protein has been shown to interact with Huntingtin and methyl CpG binding protein 2 (MeCP2). Alternative splicing results in different transcript variants.

Gene Expression Validation

No.	Sample	hPRPF40B -1CтMean	β-h_actinCтMean	ΔCтMean	ΔΔCт	Expression Rate
1	PRPF40B Stable Expression Cell Line (Hela)	18.748	13.712	5.036	-6.320	79.893
2	hela_Ctrl(Puro)	25.037	13.681	11.356	0.000	1.000

STR Profile

Download STR Report

Loci	STR Info (Sample Cell) Sample Cell Line:Hela			STR Info (Cell bank) Cell Line:HELA
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	12		11	12
D13S317	12	13.3		12	13.3
D7S820	8	12		8	12
D16S539	9	10		9	10
VWA	16	18		16	18
TH01	7	7		7	7
AMEL	X	X		X	X
TPOX	8	12		8	12
CSF1PO	9	10		9	10
D12S391	20	24	25.0
FGA	21	21
D2S1338	17	17
D21S11	27	28
D18S51	16	16
D8S1179	12	13
D3S1358	15	18
D6S1043	18	19
PENTAE	7	17
D19S433	13	14
PENTAD	8	15
D1S1656	12	15

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

High expression

Exclusive cell selection technique, ensuing high expression, RT-qPCR validation data provided.
Good stability

Stable gene copy number, low passage, can be stably passaged for more than 20 passages.
Strict QC

Strict quality control, ensuing no bacterial and mycoplasma contamination, STR authentication and cell post-thawing viability test passed; High standard delivery.

Citations:

[1] Li, Dan, et al. "SARS-CoV-2 receptor binding domain radio-probe: a non-invasive approach for angiotensin-converting enzyme 2 mapping in mice."?Acta Pharmacologica Sinica?43.7 (2022): 1749-1757.

[2] Ying, Zhenguang et al. “CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72.” BMC biology vol. 20,1 240. 24 Oct. 2022,

[3] Zhao, Chunyuan, et al. "Polyamine metabolism controls B-to-Z DNA transition to orchestrate DNA sensor cGAS activity." Immunity (2023).

[4] Xiong, Rui. The Degradation Mechanisms of Aryl Hydrocarbon Receptor in Human Lung Epithelial Carcinoma A549 Cells. Diss. University of the Pacific, 2023.

Product Catalog#：YOE-L072

Product Name：PRPF40B Stable Expression Cell Line (Hela)

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